:78 Function create_function() is deprecated [8192]
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diff --git a/README b/README index b77f87b..2f4a236 100644 --- a/README +++ b/README @@ -483,6 +483,32 @@ where the file "sample.input" contains the following files: alnSRX483017_1.fastq.gz-sorted.bam +IV.iv) Full analysis of Oxytricha trifallax + We describe the ste-by-step case of how to analyze the data for the Oxytricha trifallax + + # 1) create a directory where allocate the data + mkdir oxy + cd oxy + + # 2) use the auxiliary tools we provide to download the datasets for Oxytricha trifallax + # 2.i) first the reference genome + PATHtoRACSrepo/datasets/get.... + # 2.ii) now the ChIP-seq data, you will the NCBI-SRA toolkit for this step! + PATHtoRACS/repo/datasets/get... + + # 3) determination of ORFs with RACS + PATHtoRACSrepo/core/countReads.sh SRX483016_1.fastq.gz SRX483017_1.fastq.gz Oxytricha_trifallax_022112_assembly.fasta Oxytricha_trifallax_022112.gff3 /dev/shm/ 16 PATHtoRACS/core/defns/OXY_gene.idf + + # 4) determination of the IGRs + # 4.i) first, cd into the ORF_... directory generated by the previous step where the results from the ORF part of the RACS pipeline were placed + cd ORF_... + # 4.ii) create the "sample.input" file containing the bames of the *.fastq.gz files to be processed, eg. + ls -1 *fastq.gz-sorted.bam > sample.input + # 4.iii) run the IGR determination part of the RACS pipeline + PATHtoRACSrepo/core/intergenic/det-interGenes.sh FINAL.table.SRX483016_1-SRX483017_1 Oxytricha_trifallax_022112.gff3 interGENs_run1_OXY.csv sample.input + + + V) comparison to MACS and Other Tools --- in prep. ---