:78 Function create_function() is deprecated [8192]
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diff --git a/README b/README index 1662688..0f0869b 100644 --- a/README +++ b/README @@ -435,7 +435,7 @@ III') normalization and cut-off without a negative control --- in prep. --- -IV) Proccessing different organisms than Tetrahymena Thermophila +IV) Proccessing generic organisms/terms, other than Tetrahymena Thermophila IV.i) Reference table manipulation During the analysis and determination of ORF, the reference file for @@ -449,14 +449,25 @@ filters have to be specified; eg. For instance, the following command will select the micro-RNA for the Tetrahymena Thermophila, - PATHtoRACS/core/table.sh T_thermophila_June2014.gff3 PATHtoRACS/core/defns/TT_mRNA.id + PATHtoRACSrepo/core/table.sh T_thermophila_June2014.gff3 PATHtoRACS/core/defns/TT_mRNA.id or, the "genes" for the Oxytricha Trifallax, - PATHtpRACS/core/table.sh Oxytricha_trifallax_022112.gff3 PATHtoRACS/core/defns/OXY_gene.sh + PATHtoRACSrepo/core/table.sh Oxytricha_trifallax_022112.gff3 PATHtoRACS/core/defns/OXY_gene.id IV.ii) ORF determination + For analizing generic organisms and terms, we need to propagate the infromation +passed to the "table.sh" script as explaied in the previous edamples for the analysis of +ORFs, + + PATHtoRACSrepo/core/countReads.sh data2/_1_MED1_INPUT_S25_L007_R1_001.fastq.gz data2/_3_MED1_IP_S27_L007_R1_001.fastq.gz T_thermophila_June2014_assembly.fasta T_thermophila_June2014.gff3 /dev/shm/ 16 PATHtoRACS/core/defns/TT_mRNA.id + + PATHtoRACSrepo/core/countReads.sh SRX483016_1.fastq.gz SRX483017_1.fastq.gz Oxytricha_trifallax_022112_assembly.fasta Oxytricha_trifallax_022112.gff3 /dev/shm/ 16 PATHtoRACS/core/defns/OXY_gene.idf + +where "SRX483017_1.fastq.gz" corresponds to the data for run #1 ChIP-Seq, and +"SRX483016_1.fast1.gz" corresponds to the data for the Input of the +Oxytricha trifallax ChIP-Seq run #1. IV.iii) IGR determination