:78 Function create_function() is deprecated [8192]
diff --git a/hpc/IGR_RACS-template.sh b/hpc/IGR_RACS-template.sh new file mode 100644 index 0000000..bcbcf6b --- /dev/null +++ b/hpc/IGR_RACS-template.sh @@ -0,0 +1,32 @@ +#!/bin/bash + +# Shell script template for running the IGR-RACS pipeline +# +# This script will run the RACS scripts in background + +# generate sample files... +ls -1 alnExperimentA_*1*.bam alnExperimentA_*2*.bam > sample.12 +ls -1 alnExperimentB_*3*.bam alnExperimentB_*4*.bam > sample.34 +ls -1 alnExperimentC_*5*.bam alnExperimentC_*6*.bam > sample.56 +ls -1 alnExperimentD_*7*.bam alnExperimentD_*8*.bam > sample.78 + +# define useful environment variables... +REFfile="PATHtoREFS/organismREFfile.gff3" +RACSscript="PATHtoRACS/core/intergenic/det-interGenes.sh" + +# run RACS-intergenics +$RACSscript \ + FINAL.table.ExperimentA_1_prot1-1_INPUT_S1_R1_001-ExperimentA_2_prot1-1_ChIP_S2_R1_001 \ + $REFfile interGENs_1-2.csv sample.12 & + +$RACSscript \ + FINAL.table.ExperimentB_3_prot1-2_INPUT_S3_R1_001-ExperimentB_4_prot1-2_ChIP_S4_R1_001 \ + $REFfile interGENs_3-4.csv sample.34 & + +$RACSscript \ + FINAL.table.ExperimentC_5_prot2_INPUT_S5_R1_001-ExperimentC_6_prot2_ChIP_S6_R1_001 \ + $REFfile interGENs_5-6.csv sample.56 & + +$RACSscript \ + FINAL.table.ExperimentC_7_prot3_INPUT_S7_R1_001-ExperimentD_8_prot3_ChIP_S8_R1_001 \ + $REFfile interGENs_7-8.csv sample.78 & diff --git a/hpc/ORF_RACS-template.sh b/hpc/ORF_RACS-template.sh new file mode 100644 index 0000000..b1d4182 --- /dev/null +++ b/hpc/ORF_RACS-template.sh @@ -0,0 +1,40 @@ +#!/bin/bash +# +# Shell script template that can be used for running the ORF-RACS pipeline in a +# set of different experiments. +# +# This script will run the RACS scripts in background, which is achieved by +# including the "&" symbol at the end of each call to the RACS script. + + +# define location of the RACS pipeline and script to run, in this case "countReads.sh" +RACSscript="PATHtoRACS/core/countReads.sh" + +# define location of data and reference files +dataDIR="PATHtoDATA/ExperimentA/run1" +refsDIR="PATHtoREFS/" + +# specify reference and annotation files +refsFILE="organismREFfile.gff3" +fastaFILE="organismANNOTATIONfile_assembly.fasta" + + +# run pipeline +$RACSscript \ + # ChIP file + $dataDIR/ExperimentA_1_prot1-1_INPUT_S1_R1_001.fastq.gz \ + # INPUT file + $dataDIR/ExperimentA_2_prot1-1_ChIP_S2_R1_001.fastq.gz \ + # reference files + $fastaFILE $refsDIR/$refsFILE \ + # working space + /dev/shm & + +$RACSscript \ + $dataDIR/ExperimentA_INPUT_S2_R1.fastq.gz \ + $dataDIR/ExperimentA_ChIP_S2_R1.fastq.gz \ + $fastaFILE $refsDIR/$refsFILE \ + /scratch1/mponce/ & + +# . . . +